How to making spore syringe without pressure cooker

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How To Making Spore Syringe Without Pressure Cooker. To make a spore book, you will need two pieces of your chosen material cut to the same size, washed well, and wiped with alcohol. Pour the desired amount of distilled water (depending on how many spore syringes you are planning to make) into the pressure cooker. Wipe the lid of the jar with alcohol wipes. Squirt alcohol back into bottle and suck up boiling water into syringe, squirt into sink, suck up more boiling water, squirt into sink, repeat about 6 or 7 times, the last time filling whole syringe with boiling water and capping it with supplied syringe cap (small plastic peice.

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Wrap the top of the jar with aluminum foil and place the jar in the pressure cooker. I�d do a little trial with the water amount. 1) pour about 200ml�s of water (around 5 ounces) into the container. Dip the tip of the syringe without the needle and pull the plunger back to suck in the water along with the spore suspensions. Insert syringe and inject 1cc of liquid culture. Remove the cap from the needle and begin to fill the shot glass filled with spores with water from your first syringe by pushing gently on the plunger.

Shake needle to spread out current mycelium.

I�d do a little trial with the water amount. With the needle still hot inject 1 cc into each jar, splitting it between two of the holes. Now the casing needs to be sterilized. Let the water cool down inside the pressure cooker. You don’t need to take the whole print, but the amount you take will change the concentration of your spore syringe. Pour 30 ml of water into it.

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Give your spore syringe a good shake and heat the needle with the torch until it is just turning red. I�d do a little trial with the water amount. After filling a syringe, seal them using the needle and its cap. You can buy a mushroom spore syringe online, or make one yourself. Flame sterilize needle until it is glowing red.

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Wipe the lid of the jar with alcohol wipes. Slowly bring it up to 15 psi. With the needle still hot inject 1 cc into each jar, splitting it between two of the holes. Put the jars, in the pressure cooker. Repeat this for each jar, heating the needle between every jar.

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Shake the syringe well, then insert the needle all the way into one of the holes. 1) pour about 200ml�s of water (around 5 ounces) into the container. Let the water cool down inside the pressure cooker. Thoroughly wipe down the with alcohol, then spray the entire area liberally. This will suspend the spores.

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Let the water cool down inside the pressure cooker. Slowly bring it up to 15 psi. Draw up the water and then immediately recap the syringe without touching the opening or syringe cap. Don the dust mask and a pair of latex gloves. A pressure cooker (or stovetop and pot) a spore print method:

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Slowly bring it up to 15 psi. Sterilize the flask or glass bottle Bring the syringe into the glove box, and get ready to crack open a petri with your left hand. Steps in making a spore syringe. Suspend the spores in water:

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Repeat for the other three holes, totaling about 1cc of spore solution per jar. Close the spore book and securely seal the other three edges with masking tape as well. Use some masking tape to make a hinge along one edge. Find a small (preferably at least partially enclosed) surface to work on. › repeat the process until you have filled every syringe you have.

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Once empty, put the syringe needle into the water spore mixture and pull. Put the jars, in the pressure cooker. Draw up the water and then immediately recap the syringe without touching the opening or syringe cap. Suspend the spores in water: Pour the desired amount of distilled water (depending on how many spore syringes you are planning to make) into the pressure cooker.

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This will suspend the spores. Put the jars, in the pressure cooker. Once empty, put the syringe needle into the water spore mixture and pull. Tilt the syringe so the needle tip touches the side of the jar. Add a measured amount of water.

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Draw 10ml of water into your syringe: Insert syringe and inject 1cc of liquid culture. A pressure cooker (or stovetop and pot) a spore print method: Drop a single drop of spore solution onto the agar. Pour the desired amount of distilled water (depending on how many spore syringes you are planning to make) into the pressure cooker.

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I�d do a little trial with the water amount. Inject approximately ¼ cc (¼ ml) of spore solution. Repeat this for each jar, heating the needle between every jar. Sterilize the flask or glass bottle use a flask or a glass bottle with a narrow opening to reduce the chances of contamination or microbes getting inside it. Insert syringe and inject 1cc of liquid culture.

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Dip the tip of the syringe without the needle and pull the plunger back to suck in the water along with the spore suspensions. Add a measured amount of water. After filling a syringe, seal them using the needle and its cap. Inject approximately ¼ cc (¼ ml) of spore solution. Squirt a few drops out of the syringe as “waste”.

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You should hear a hiss as the needle cools. Sterilize the flask or glass bottle use a flask or a glass bottle with a narrow opening to reduce the chances of contamination or microbes getting inside it. Draw up the water and then immediately recap the syringe without touching the opening or syringe cap. Repeat for the other three holes, totaling about 1cc of spore solution per jar. Tilt the syringe so the needle tip touches the side of the jar.

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